Mastering the Narishige PC-10: A Comprehensive Guide to the Dual-Stage Pipette Puller
This guide serves as a manual for new users, detailing its features, setup, and optimization strategies to ensure your research stays precise.
Press the Start switch. The machine will automatically perform the first pull, wait, and then execute the second, final pull. 5. Optimizing Pulling Settings
: Approximately 80W, compatible with various AC voltages (100V–240V) depending on internal settings.
Clean your glass capillaries with ethanol and dry them thoroughly to remove finger oils and dust. Dust particles cause asymmetric breaks during the pull. Step 2: Install the Capillary narishige pc10 manual new
Turn on the main power switch and allow the electronics to stabilize for 5 minutes.
: Includes two types of weights (light and heavy) for fine tension adjustment. Technical Data Heater Level : 100 = 2.5V heater voltage. Power Source : 100V–240V AC (±5%). Dimensions : W205 x D190 x H185mm; Weight: Approx. 6.0kg. 2. Safety & Handling Precautions
Periodically clear out glass shards from the bottom catch tray to prevent mechanical jams.
This comprehensive manual provides the necessary steps to set up, calibrate, operate, and troubleshoot your Narishige PC-10 to ensure reproducible results in your laboratory. 1. Safety and Installation Guidelines Mastering the Narishige PC-10: A Comprehensive Guide to
The process stops after an initial "softening" pull and then performs a second, more intense pull. This is essential for producing firm microelectrodes with the sharp tips (0.1µm to several µm) required for patch clamp experiments. 3. Setting Your Parameters
| Problem | Likely Cause | Fix | |--------|-------------|-----| | Glass melts but does not pull | Magnet power too low | Increase magnet power | | Glass breaks into pieces | Too much heat or too fast pull | Lower heater current or reduce weight | | Tip too long & floppy | Insufficient heat | Increase heater current or timer | | Pipette bent at tip | Uneven heating | Balance left/right heater currents | | No heating | Blown heater coil | Replace coil (order from Narishige) |
: Install on a level, horizontal surface in a non-humid environment. Maintenance
: Use the NO. 1 and NO. 2 heater adjusting knobs to set specific heat levels for each stage. Dust particles cause asymmetric breaks during the pull
You will also need to order consumable glass capillaries. The compatible glass types include:
Loosen terminal blocks and realign the coil loop so it is equidistant from the glass on all sides. Check for uneven room drafts. The heating coil is warped or oxidized unevenly.
Clean the filament, ensure the capillary is aligned perfectly. 7. Conclusion
Every time you install a new platinum heating coil, you must determine its specific "burn value" (the minimum current required to melt the glass).