Eppendorf 5402 Manual Updated 🎉
Press or turn the temperature control to your target setpoint (commonly 4°C for DNA/RNA or protein extractions). Allow the unit to pre-cool if necessary.
The updated manual now includes a mandatory replacement of the rubber motor mounts if the centrifuge is used for qPCR prep (low vibration requirement). The old rubber compound hardens and transmits vibration after 8 years.
: Use a damp, lint-free cloth with diluted neutral detergents or alcohol. Avoid caustic chemicals that can corrode the aluminum rotor. Condensation
Adjust the speed knob or arrow buttons until the desired RPM or g-force is shown on the digital display. eppendorf 5402 manual updated
The Ultimate Guide to the Eppendorf 5402 Centrifuge: Operation, Maintenance, and Updated Manual Resources
18 × 1.5 mL / 2.0 mL microcentrifuge tubes
Screw the aerosol-tight rotor lid securely onto the rotor spindle. Operating the unit without the rotor lid can cause high-pitched wind noise and compromises sample containment. Running the Centrifuge Press or turn the temperature control to your
(not the user manual)?
Proper preventive maintenance extends the lifespan of the Eppendorf 5402 and ensures accurate, repeatable experimental results. Daily Care
The Eppendorf 5402 was designed as a compact, refrigerated centrifuge capable of speeds up to 14,000 rpm (approximately 20,800 x g). Its primary applications included: The old rubber compound hardens and transmits vibration
Stop the run immediately. Re-balance tubes. If it persists empty, the motor mounts need replacement. Blown fuse or faulty power cord.
Operating laboratory equipment safely requires strict adherence to technical limitations. Below are the core technical specifications for the Eppendorf 5402. 14,000 RPM (Rotations Per Minute) Maximum RCF: 16,000 × g (Relative Centrifugal Force) Temperature Range: -9°C to +40°C
